A new assay capturing chromosome fusions shows a protection trade-off at telomeres and NHEJ vulnerability to low-density ionizing radiation
نویسندگان
چکیده
Abstract Chromosome fusions threaten genome integrity and promote cancer by engaging catastrophic mutational processes, namely chromosome breakage–fusion–bridge cycles chromothripsis. are frequent in cells incurring telomere dysfunctions or those exposed to DNA breakage. Their occurrence therefore their contribution instability unchallenged is unknown. To address this issue, we constructed a genetic assay able capture quantify rare budding yeast. This fusion (CFC) relies on the controlled inactivation of one centromere rescue unstable dicentric fusions. It sensitive enough basal rate end-to-end occurring wild-type cells. These depend canonical nonhomologous end joining (NHEJ). Our results show that protection from trade-off at telomeres between positive effectors (Rif2, Sir4, telomerase) negative effector partially antagonizing them (Rif1). The CFC also captures NHEJ-dependent induced ionizing radiation. provides evidence for chromosomal rearrangements stemming single photon–matter interaction.
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2021
ISSN: ['1362-4962', '1362-4954', '0305-1048']
DOI: https://doi.org/10.1093/nar/gkab502